![]() Concerning genome engineering approaches based on nuclease-induced DNA double-strand breaks, this protocol could aid in detecting the unwanted effects caused by the donor fragments themselves. There are currently two (2) licenses available for use. CLC can currently be accessed from Prairie Thunder or Iris, our stand-alone, non-managed cluster nodes. This work should pave the way for future genotoxicity analyses. CLC Bio is a comprehensive analysis package for the analysis and visualization of data from next-generation sequencing. Additionally, we demonstrated the usefulness of this approach for primary cells by treating human CD34 + hematopoietic stem and progenitor cells with a 100-nucleotide-long unmodified oligodeoxynucleotide directed against the endogenous CYBB locus. For a 21-nucleotide-long phosphorothioate-modified oligodeoxynucleotide, we compiled a broad array of error-free incorporations, point mutations, indels, and structural rearrangements from actively dividing HEK293-derived cells. This protocol was validated in gene repair experiments without intentionally inducing a DNA double-strand break. Affected chromosomal fragments are enriched and preferably sequenced by nanopore sequencing. Price from 9.99 to 1999. Thus, we have developed a protocol that follows the fate of a biotin-labeled single-stranded oligodeoxynucleotide in human cells based on its physical incorporation into the targeted genome. Because the usability of single-stranded oligodeoxynucleotides depends on their efficiencies, as well as their specificities, analyzing their genotoxic off-target activities is important. They enable gene repair and genome editing, and they are central to the antisense technology. ![]() Short single-stranded oligodeoxynucleotides are versatile molecular tools used in different applications. ![]()
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